By John Swansbury
A set of key cytogenetic and FISH ideas utilized by sleek medical laboratories within the genetic research of human malignancies. The book's sensible suggestion and strategies are appropriate to be used at each point of workmanship, together with totally validated laboratories, yet with a sympathetic bias in the direction of somebody contemplating developing a brand new cytogenetics provider. right here the reader will locate not just simple tutorials at the basics of human karyotypes and chromosome research, but additionally targeted discussions on how laboratories may perhaps optimally improve their repertoire of functions to incorporate such more recent complementary ideas as CGH, FISH, and M-FISH.
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Extra resources for Cancer Cytogenetics: Methods and Protocols (Methods in Molecular Biology)
Stock, A. , Dennis, T. , and Spallone, P. A. 1) chromosomal breakpoints associated with acute promyelocytic leukemia. Cancer Genet. Cytogenet. 119, 15–17. 23. , et al. (1998) The importance of diagnostic cytogenetics on outcome in AML: analysis of 1,612 patients entered into the MRC AML 10 trial. Blood 92, 2322–2333. 24. , Larson, R. , and Rowley, J. D. (1993) Persistence of the 8;21 translocation in patients with acute myeloid leukemia type M2 in long-term remission. Blood 82, 712–715. 25. Hiorns, L.
Refractory anemia ± sideroblasts: < 10% dysplastic granulocytes. 2. Cytopenia: May have bilineage or trilineage dysplasia but < 5% blasts. 3. RAEB 1: With 5–10% blasts. 4. RAEB 2: With 11–19% blasts. 5. CMML in either MDS or MPD. 6. 5q-syndrome. Note that the RAEBt class has been abolished, such that the presence of 20% blasts now defines acute leukemia. Like the MPDs, most of the MDSs are usually slow-evolving disorders in which supportive treatment may be adequate in the early stages; aggressive cytotoxic treatment rarely produces a remission and is more likely to induce bone marrow failure or acceleration of disease progression.
If the laboratory has access to a cell counter, then it is worth using it to identify those cases with particularly high or low cell counts. It is easy to over-inoculate cultures from patients with chronic myeloid leukemia (CML) and occasionally other types of leukemia if the white cell count is very high; the final dilution should be about 1–2 × 106/mL. Adding too many cells usually results in failure to obtain any useful divisions at all. Underinoculation is less serious, but if the cell count is very low then use smaller tubes and set up 5-mL or 2-mL cultures.
Cancer Cytogenetics: Methods and Protocols (Methods in Molecular Biology) by John Swansbury