By E. P. Abraham, G. G. F. Newton (auth.), David Gottlieb, Paul D. Shaw (eds.)
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And ~ 28 HRS. -:;'------___ the growth period. Chloramphenicol ~IOO synthesis generally paralled growth, /~ but about 12 hr. 3). DED AT natural substrate. / The amount of antibiotic that can be 20 40 60 80 100 HOURS synthesized in a specific medium, by a Fig. 4. The effect of the addition of chloramphenicol at various time intervals strain of S. venezuelae, seems fixed and during the growth of S. venezuelae on the probably is regulated by a feedback subsequent production of the antibiotic mechanism (LEGATOR and GOTTLIEB, 1953).
220,477 (1956). STRATTON, CH. , and M. C. REBSTOCK: A new metabolite of Streptomyces venezuelae D-threo-1-p-aminophenyl-2-dichloroacetamido-1 ,3-propanediol. Arch. Biochem. Biophys. 103, 159 (1963). , T. TAZAKI, H. OKAMI, and S. FUKUYAMA: Isolation of a crystalline antibiotic substance from a strain of streptomyces and its identity with chloromycetin. Japan. Med. J. 1, 358 (1948). VINING, L. , and D. W. S. WESTLAKE: Biosynthesis of the phenylpropanoid moiety of chloramphenicol. Can. J. Microbiol.
Incorporation by S. 90 * S. 450 mmole of phenylalanine was added to 300 ml of media. ** Specific activity, 422 fLcfmmole. *** Specific activity, 222 fLcfmmole. , 1962). The incorporation into chloramphenicol of variously labeled phenylanines as _P4C, -2-14C, -3-14C and -U-14C resulted in no significant labeling of the corresponding aryl or serinol carbons (Table 2). Almost all the radioactivity from _P4C and _2_14C was in the carboxyl group of the dichloracetyl portion of the molecule. With -3-14C minor labeling was located in the pNPS portion but again the dichloracetyl was more higly active; however, almost 90% was in the dichlormethyl carbon.
Biosynthesis by E. P. Abraham, G. G. F. Newton (auth.), David Gottlieb, Paul D. Shaw (eds.)