Biomolecular Engineering in the European Community: by E. Magnien (auth.), E. Magnien (eds.) PDF

By E. Magnien (auth.), E. Magnien (eds.)

ISBN-10: 9400944667

ISBN-13: 9789400944664

ISBN-10: 9401084882

ISBN-13: 9789401084888

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Extra info for Biomolecular Engineering in the European Community: Achievements of the Research Programme (1982 – 1986) — Final Report

Example text

0 ~ ~ ~ i u § 'i! 5 2 Fig. 3 . Variation of electrophoretic mobility of s. 2 mM, O. suspension in AI(N0 3 )3 0 . 37 mM; open symbols strain D273-10B ; black symbols, strain IL12S-B. This figure illustrates the influence of pH and AI 3+-ions on the surface charge. The cell hydrophobicity determines the extent to which the electrostatic repulsions can be overcome simply by strength. Hydrophobic cells can on a support. according to the Hydrophilic cells results using an adequate pH in this way always be forced to need of Rouxhet.

Gasson). These vectors may also be used in conjonction with B. subtilis and E. coli. -.. ---- 2. ~~ylis. The two main difficulties encountered in expressing a cloned DNA fragment on an industrial scale are the instability of the recombinant molecule and the deleterious effect overproduction may have on the cells themselves. Thus, work in Dublin (D. ~~' Firstly, stable cryptic plasmids were isolated from industrial Bacilli strains and used to construct cloning vectors. Sequences involved in conferring stability were then identified and are being characterized by deletion analysis and DNA sequencing.

Yeasts. ~.! cerevisiae strains with new fermenting properties. lL~!!. , Kluyveromyc~.! ~.! (which are unsuitable for alcoholic fermentation) will degrade these compounds. C. Emeis (Aachen) investigated the possibility to transfer the corresponding genetic material to the ~accharomyces and in order to do so, developed the techniques necessary for somatic hybridization. He was able to make protoplasts from all of the strains involved and protoplast regeneration was succesfully achieved. Stable hybrids were recovered from the PEGinduced protoplast fusion of S.

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Biomolecular Engineering in the European Community: Achievements of the Research Programme (1982 – 1986) — Final Report by E. Magnien (auth.), E. Magnien (eds.)


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